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Hyperbaric oxygen therapy protects the liver from apoptosis caused by ischemia‐reperfusion injury in rats

2009; Wiley; Volume: 29; Issue: 7 Linguagem: Inglês

10.1002/micr.20664

1098-2752

José Carlos Chaves, Djalma José Fagundes, Manuel de Jesus Simões, Paulo Roberto Bertoletto, Celina T. F. Oshima, Murched Omar Taha, Ricardo Santos Simões, Anna Luiza Negrini Fagundes,

Mesenchymal stem cell research

Abstract Purpose: The present paper aimed to investigate the role of hyperbaric oxygen treatment (HBO) and the apoptosis in rat liver ischemia‐reperfusion injury (IRI). Methods: Thirty‐seven male Wistar rats were subjected to 30 minutes of hepatic ischemia and 30 minutes of reperfusion and randomly distributed into six groups: G‐I/R ( n = 8), control without HBO; G‐HBO/I ( n = 8), HBO only during the ischemia period; G‐HBO/R ( n = 8), HBO only during the reperfusion period; G‐HBO‐I/R ( n = 8), HBO during both the ischemia and reperfusion periods; G‐Sh ( n = 3), HBO without ischemia or reperfusion as sham group; G‐C ( n = 2) for control of current apoptosis expression on the normal liver tissue. HBO was carried out using a transparent, cylindrical acrylic chamber with a pressure of 2.0 ATA. Hepatic samples were stained for caspase‐3 cleavage. Results: Apoptotic cells were identified in all groups. In the hepatic specimens of animals HBO‐treated during ischemia (GHBO‐I), there was a significant decrease ( P < 0.001) in the number of cells undergoing apoptosis (1.62 ± 0.91). The apoptotic index showed no significant difference in the animals HBO‐treated during ischemia/reperfusion (5.75 ± 1.28) compared with the G‐I/R (3.5 ± 0.75), which had no HBO treatment. The apoptosis index (11.25 ± 1.90) was significantly higher ( P < 0.01) in HBO‐treated animals during the reperfusion period when compared with any of the other groups. Conclusion: A favorable effect was obtained when hyperbaric oxygen was administered early during ischemia. The hyperbaric oxygen in later periods of reperfusion was associated with a more severe apoptosis index. © 2009 Wiley‐Liss, Inc. Microsurgery 2009.