Affinity chromatography of soluble galactosyltransferases using an easily synthesized N-acetylglucosamine-agarose bead
1976; Birkhäuser; Volume: 32; Issue: 6 Linguagem: Inglês
10.1007/bf01919834
ISSN0014-4754
AutoresEric G. Berger, Milton M. Weiser, Kurt J. Isselbacher,
Tópico(s)Microbial Metabolic Engineering and Bioproduction
ResumoAgarose derivatized withp-nitrophenyl-N-acetyl-β-d-glucosamine was used for affinity chromatography for soluble galactosyltransferase from various sources. Moreover, this adsorbant acts as an acceptor for galactose when incubated with prepurified galactosyltransferase.
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