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Studies on a possible molecular basis for the structure of mitochondrial cristae

1988; Wiley; Volume: 1; Issue: 1 Linguagem: Inglês

10.1002/jmr.300010105

1099-1352

Balázs Sümegi, Dale A. Freeman, Lindsey Inman, Paul A. Srere,

Metabolomics and Mass Spectrometry Studies

Abstract We have investigated a possible molecular basis for mitochondrial cristae formation. Proteoliposomes containing electron transport proteins, cytochrome oxidase, or complex III in their proper orientation bind to pig heart mitoplasts but not pig heart mitochondria. Using Leydig tumor cells, we have confirmed earlier reports that chloramphenicol causes a diminution in cristae content and a change in its characteristic lamellar form. We show that the proteoliposomes containing cytochrome oxidase or complex III in the proper orientation bind to mitoplasts from Leydig tumor cells but do not bind as well to mitoplasts from chloramphenicol‐treated Leydig tumor cells. These experiments provide a possible mechanism to explain cristae formation.