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Thiamin Biosynthesis in Eukaryotes: Characterization of the Enzyme-Bound Product of Thiazole Synthase from Saccharomyces c erevisiae and Its Implications in Thiazole Biosynthesis

2006; American Chemical Society; Volume: 128; Issue: 22 Linguagem: Inglês

10.1021/ja061413o

1943-2984

Abhishek Chatterjee, Christopher T. Jurgenson, Frank C. Schroeder, S.E. Ealick, Tadhg P. Begley,

Biochemical and Molecular Research

The biosynthesis of thiamin pyrophosphate in eukaryotes is different from the prokaryotic biosynthesis and is poorly understood to date. Only one thiazole biosynthetic gene has been identified (Thi4 in Saccharomyces cerevisiae). Here we report the identification and characterization of a Thi4-bound metabolite that consists of the ADP adduct of 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid. The unexpected structure of this compound yields the first insights into the mechanism of thiamin thiazole biosynthesis in eukaryotes.