Produção Nacional
Revisado por pares
Immunodiagnosis of swine cysticercosis by indirect ELISA employing a heterologous antigen from Taenia crassiceps metacestode
1996; Elsevier BV; Volume: 64; Issue: 4 Linguagem: Inglês
10.1016/0304-4017(95)00928-0
ISSN1873-2550
AutoresGermano Francisco Biondi, R G Mucciolo, Cáris Maroni Nunes, Leonardo José Richtzenhain,
Tópico(s)Toxoplasma gondii Research Studies
ResumoC. Larralde et al. (1990, Arch. Pathol. Lab. Med., 114:926–928) demonstrated that heterologous antigen from the laboratory-adapted murine Taenia crassiceps metacestode may substitute those from Taenia solium in the immunodiagnosis of human cysticercosis by the indirect enzyme-linked immunosorbent assay (IE). This antigen is easily obtained at a laboratory level and solves the problem of T. solium cysticerci collection from naturally or experimentally infected swine. In this study an IE employing a heterologous antigen from the T. crassiceps metacestode was evaluated for the immunodiagnosis of swine cysticercosis. Sera from 300 swine free of T. solium cysticerci by post-mortem examination were employed to determine two IE cut- off values: 1) Mean ELISA values + 2 standard deviations (2σ cut-off) and 2) - Mean ELISA values + 3 standard deviations (3σ cut-off). The specificity of IE was 97% with the 2σ cut-off and 100% with the 3σ cut-off. When applied to ten sera from swine infected by cysticerci of T. solium by post-mortem examination, the sensitivity of IE was 100% independent of the cut off.
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