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Anti-loxoscelic horse serum produced against a recombinant dermonecrotic protein of Brazilian Loxosceles intermedia spider neutralize lethal effects of Loxosceles laeta venom from Peru

2014; Elsevier BV; Volume: 93; Linguagem: Inglês

10.1016/j.toxicon.2014.10.023

1879-3150

Clara Guerra-Duarte, César Bonilla Ferreyra, Gabriela Guimarães, Ricardo Andrez Machado‐de‐Ávila, Thais Melo Mendes, W. Silva, B. Tintaya, Armando Yarlequé, Carlos Chávez-Olórtegui,

Insect and Pesticide Research

In this work, an anti-loxoscelic serum was produced by immunizing horses with a recombinant dermonecrotic protein from Loxosceles intermedia (rLiD1). Anti-rLiD1 antibodies were able to recognize different species of Loxosceles venoms by Western Blot and ELISA. The efficacy of anti-rLiD1 serum against the toxic effects of Loxosceles laeta (Peru) venom was tested, showing that anti-rLiD1 serum can neutralize those effects. This study confirms that recombinant proteins can be good candidates to replace crude venoms for antivenom production.