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Development of enzyme immunoassay for detection of DDT

2007; Taylor & Francis; Volume: 43; Issue: 1 Linguagem: Inglês

10.1080/03601230701734964

1532-4109

Masashi Hirano, Kazuyuki Kitamura, Ikuo Kato, Chizuko Yanaihara, Ken‐ichi Iwamoto, Makiko Sekiyama, Chiho Watanabe, Takashi Nakamoto, Nobukazu Miyamoto, Yuta Onishi, Koji Arizono,

Analytical chemistry methods development

Abstract Dichlorodiphenyltrichloroethane (DDT) is one of the persistent organic pollutants (POPs) widely found in the environment and in the general population. In this study, a direct competitive enzyme immunoassay (EIA) has been developed for the quantitative analysis of DDT. To generate a specific polyclonal antibody for EIA, p, p′-DDT was conjugated to porcine thyroglobulin for rabbit immunization. At optimized EIA conditions, the standard curves ranged from 0.137 to 100 ng/mL with the quantification limit of 0.41 ng/mL. The coefficients of variation (CV%) were 5.42–10.53% for intra-assay and 6.04–7.26% for inter-assay. Cross-reactivities with DDT metabolites (DDTs, including o, p′-DDT, p, p′-DDD, o, p′-DDD, p, p′-DDE, o, p′-DDE, p, p′-dichlorobenzophenone (DCBP), o, p′-DCBP) were investigated. The polyclonal antibody showed relatively low and/or no cross-reactivity with these compounds, and the assay was seen to be highly selective for p, p′-DDT. Moreover, the DDTs could be ranked by their reactivity: DDT > DDD > DDE > DCBP. In addition, the characterization of the polyclonal antibody indicated that the antiserum possesses a high specificity for p, p′-isomers. The results indicated that the developed EIA using this antibody could be a convenient and supplemental analytical tool for monitoring DDT. Keywords: EIADDTpolyclonal antibodymonitoring Acknowledgments This paperwas presented at the SETACAsia/Pacific 2006 conference held at Peking University, China on September 18-20, 2006. Notes a SD: standard deviation. b CV: coefficient of variation.