The photochromic properties of 4-keto bacteriorhodopsin
1996; Elsevier BV; Volume: 6; Issue: 4 Linguagem: Inglês
10.1016/s0925-3467(96)00050-x
ISSN1873-1252
AutoresTimo Jääskeläinen, V.P. Leppanen, Sinikka Parkkinen, Jussi Parkkinen, А. А. Ходонов,
Tópico(s)Retinal Development and Disorders
ResumoThe all-trans-retinal of the native chromoprotein of bacteriorhodopsin (BR) purple membrane was replaced with the synthetic 4-keto retinal. Gelatin films were made from the reconstituted BR and the intensity-dependent basic optical properties were investigated. It is shown that at room temperature the M-state is composed of two different absorption bands (maximum at 413 nm and 435 nm) with different relaxation times. Unexpected low-intensity transmission properties which cannot be explained by the two-level model are reported. The M-state of the 4-keto BR is shown to be about 100 times slower than the M-state of the similar wild type BR-film.
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