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Analysis of Neurotoxin 3- N -Oxalyl- l -2,3-diaminopropionic Acid and Its α-Isomer in Lathyrus sativus by High-Performance Liquid Chromatography with 6-Aminoquinolyl- N -hydroxysuccinimidyl Carbamate (AQC) Derivatization

2000; American Chemical Society; Volume: 48; Issue: 8 Linguagem: Inglês

10.1021/jf000033y

1520-5118

Chen Xiong, Fei Wang, Qian Chen, Xing Chen Qin, Zhixiao Li,

Plant Molecular Biology Research

A method was developed for the quantitative determination of the neurotoxic nonprotein amino acid, 3-N-oxalyl-L-2,3-diaminopropionic acid (beta-ODAP), and its nontoxic alpha-isomer, 2-N-oxalyl-L-2, 3-diaminopropionic acid (alpha-ODAP), in the plant samples of Lathyrus sativus after derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) by reversed-phase high-performance liquid chromatography (HPLC). Hippuric acid was used as an internal standard. A linear response was recorded in the concentration rang 0.32-32 nmol with r > 0.999. The RP HPLC detection limit for both isomers is 1.8 ng. According to different experimental needs, a ternary gradient system can be used to determine toxin and other nonprotein amino acids. The RP HPLC method and a colorimetric method were compared for measuring ODAP.