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BIBTEX RIS

The orthotopic xenotransplant of human glioblastoma successfully recapitulates glioblastoma-microenvironment interactions in a non-immunosuppressed mouse model

2014; BioMed Central; Volume: 14; Issue: 1 Linguagem: Inglês

10.1186/1471-2407-14-923

ISSN

1471-2407

Autores

Celina García, Luiz Gustavo Dubois, Anna L. R. Xavier, Luiz Henrique Geraldo, Anna Carolina Carvalho da Fonseca, Ana Helena Pereira Correia, Fernanda Meirelles, Grasiella M. Ventura, Luciana Romão, Nathalie Henriques Silva Canêdo, Jorge Marcondes de Souza, João R.L. Menezes, Vivaldo Moura‐Neto, Fernanda Tovar‐Moll, Flávia Regina Souza Lima,

Tópico(s)

Neuroinflammation and Neurodegeneration Mechanisms

Resumo

Glioblastoma (GBM) is the most common primary brain tumor and the most aggressive glial tumor. This tumor is highly heterogeneous, angiogenic, and insensitive to radio- and chemotherapy. Here we have investigated the progression of GBM produced by the injection of human GBM cells into the brain parenchyma of immunocompetent mice. Xenotransplanted animals were submitted to magnetic resonance imaging (MRI) and histopathological analyses. Our data show that two weeks after injection, the produced tumor presents histopathological characteristics recommended by World Health Organization for the diagnosis of GBM in humans. The tumor was able to produce reactive gliosis in the adjacent parenchyma, angiogenesis, an intense recruitment of macrophage and microglial cells, and presence of necrosis regions. Besides, MRI showed that tumor mass had enhanced contrast, suggesting a blood–brain barrier disruption. This study demonstrated that the xenografted tumor in mouse brain parenchyma develops in a very similar manner to those found in patients affected by GBM and can be used to better understand the biology of GBM as well as testing potential therapies.

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