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Modified E-rosette test for detection of total and active rosette-forming lymphocytes

1976; Elsevier BV; Volume: 13; Issue: 3-4 Linguagem: Inglês

10.1016/0022-1759(76)90066-1

1872-7905

Mary Jo Florey, F. Peetoom,

Glycosylation and Glycoproteins Research

The human peripheral blood T-lymphocyte population can be demonstrated with an economy of time by using bromelin-treated sheep red blood cells (SRBC). Similarity, the lower affinity, active T-cell population can be determined using untreated SRBC. Both tests are done without serum, or prolonged incubation of the cell mixture, using the same procedural steps reflecting changes as readily as longer test methods.