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The nucleotide sequence of an infectious clone of the geminivirus beet curly top virus

1986; Springer Nature; Volume: 5; Issue: 8 Linguagem: Inglês

10.1002/j.1460-2075.1986.tb04424.x

1460-2075

John Stanley, Peter Markham, Rowena Callis, Marion S. Pinner,

Viral Infections and Vectors

Article1 August 1986free access The nucleotide sequence of an infectious clone of the geminivirus beet curly top virus J. Stanley J. Stanley John Innes Institute, Colney Lane, Norwich NR4 7UH, UK Search for more papers by this author P.G. Markham P.G. Markham John Innes Institute, Colney Lane, Norwich NR4 7UH, UK Search for more papers by this author R.J. Callis R.J. Callis John Innes Institute, Colney Lane, Norwich NR4 7UH, UK Search for more papers by this author M.S. Pinner M.S. Pinner John Innes Institute, Colney Lane, Norwich NR4 7UH, UK Search for more papers by this author J. Stanley J. Stanley John Innes Institute, Colney Lane, Norwich NR4 7UH, UK Search for more papers by this author P.G. Markham P.G. Markham John Innes Institute, Colney Lane, Norwich NR4 7UH, UK Search for more papers by this author R.J. Callis R.J. Callis John Innes Institute, Colney Lane, Norwich NR4 7UH, UK Search for more papers by this author M.S. Pinner M.S. Pinner John Innes Institute, Colney Lane, Norwich NR4 7UH, UK Search for more papers by this author Author Information J. Stanley1, P.G. Markham1, R.J. Callis1 and M.S. Pinner1 1John Innes Institute, Colney Lane, Norwich NR4 7UH, UK The EMBO Journal (1986)5:1761-1767https://doi.org/10.1002/j.1460-2075.1986.tb04424.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info A number of infectious clones of a Californian isolate of the leafhopper-transmitted geminivirus beet curly top virus (BCTV) have been constructed from virus-specific double-stranded DNA isolated from infected Beta vulgaris and used to demonstrate a single component genome. The nucleotide sequence of one infectious clone has been determined (2993 nucleotides). Comparison with other geminiviruses has shown that the organisation of the genome closely resembles DNA 1 of the whitefly-transmitted members. The four conserved coding regions of DNA 1 have highly homologous counterparts in BCTV with the exception of the putative coat protein which is more closely related to those of the leafhopper-transmitted geminiviruses suggesting a strong interrelationship between coat protein and insect vector. A BCTV component equivalent to DNA 2 is not required for virus infection or transmission and has not been isolated from infected plants. Previous ArticleNext Article Volume 5Issue 81 August 1986In this issue RelatedDetailsLoading ...