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Determination of dioscin in rat plasma by liquid chromatography–tandem mass spectrometry

2005; Elsevier BV; Volume: 817; Issue: 2 Linguagem: Inglês

10.1016/j.jchromb.2004.12.026

1873-376X

Ke Li, Yingwu Wang, Jingkai Gu, Xiaohong Chen, Dafang Zhong,

Plant-based Medicinal Research

A sensitive and specific high-performance liquid chromatography–tandem mass spectrometric (LC–MS/MS) assay for dioscin in rat plasma was developed. Ginsenoside Rh2 was employed as an internal standard. Dioscin is a naturally occurring saponin present in many traditional Chinese medicinal plants. Dioscin was determined after the acetonitrile-mediated plasma protein precipitation. The mobile phase consisted of acetonitrile:10 mmol/l aqueous ammonium acetate (95:5, v:v), which was pumped at 0.8 ml/min. The analytical column (100 mm × 4.6 mm i.d.) was packed with Hypersil ODS material (5 μm). The standard curve was linear from 1 to 100 ng/ml. The assay was specific, accurate (percentage deviations from nominal concentrations were <10%), precise and reproducible (within- and between-day coefficients of variation <10%). Dioscin in rat plasma was stable over three freeze–thaw cycles and at ambient temperatures for 24 h. The utility of the assay was demonstrated by determining dioscin plasma concentrations in five rats for 120 h following a single oral gavage dose of 90 mg/kg.