Expression of Transforming Growth Factor-α-Like Messenger Ribonucleic Acid Transcripts in the Bovine Mammary Gland
1995; Elsevier BV; Volume: 78; Issue: 9 Linguagem: Inglês
10.3168/jds.s0022-0302(95)76815-1
ISSN1529-9066
Autores Tópico(s)T-cell and Retrovirus Studies
ResumoThe objective of this study was to evaluate the expression of mRNA for transfonning growth factor-a in the b0vine mammary gland.Total RNA from 23 cows at various stages of development was isolated from mammary parenchyma and subjected to Northern blot analysis using human transforming growth factor-a 32P-labeled deoxycytosine triphosphate cDNA probe.When blots were washed through Ix sodium chloride and sodium citrate plus 1% SDS at 60•C, a 4.8-kb band was observed in all RNA samples.When stringency was increased to .1x, mRNA transcripts were observed only for cows at d 233.240, and 244 of gestation.After the high stringency washes.major bands were observed at 7.4 and 3.3 kb and minor bands on d 233 at 5.4,2.3, and 1.6 kb.No additional transcripts were detected in RNA from mammary glands of nonpregnant cows during involution.lactating cows.or estrogen-treated heifers.These data indicate that transcripts similar to transfonning growth factor-a are present in the bovine mammary gland.The expression pattern could represent alternatively spliced transcripts that were produced during an important period of mammary development prior to parturition.
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