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A broad-spectrum cytolytic toxin from Bacillus thuringiensis var. kyushuensis

1992; Royal Society; Volume: 248; Issue: 1321 Linguagem: Inglês

10.1098/rspb.1992.0035

1471-2954

Barbara H. Knowles, Philip J. White, Colin N. Nicholls, David J. Ellar,

Bacillus and Francisella bacterial research

Restricted accessMoreSectionsView PDF ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmail Cite this article Knowles Barbara H. , White Philip J. , Nicholls Colin N. and Ellar David J. 1992A broad-spectrum cytolytic toxin from Bacillus thuringiensis var. kyushuensisProc. R. Soc. Lond. B.2481–7http://doi.org/10.1098/rspb.1992.0035SectionRestricted accessArticleA broad-spectrum cytolytic toxin from Bacillus thuringiensis var. kyushuensis Barbara H. Knowles Google Scholar Find this author on PubMed Search for more papers by this author , Philip J. White Google Scholar Find this author on PubMed Search for more papers by this author , Colin N. Nicholls Google Scholar Find this author on PubMed Search for more papers by this author and David J. Ellar Google Scholar Find this author on PubMed Search for more papers by this author Barbara H. Knowles Google Scholar Find this author on PubMed , Philip J. White Google Scholar Find this author on PubMed , Colin N. Nicholls Google Scholar Find this author on PubMed and David J. Ellar Google Scholar Find this author on PubMed Published:22 April 1992https://doi.org/10.1098/rspb.1992.0035AbstractBacillus thuringiensis (Bt) var. kyushuensis synthesizes a mosquitocidal crystalline inclusion containing several proteins ranging from 140 to 14 kDa. We have identified a 25 kDa protein protoxin in this inclusion which is not cytolytic, but when activated proteolytically to 23—22 kDa products is cytolytic to mosquito, lepidopteran and mammalian cells, can release entrapped glucose from liposomes and forms cationselective channels in a planar lipid bilayer. This broad-spectrum cytolytic toxin is related antigenically to the 23 kDa toxin from Bt var. darmstadiensis strain 73—E10-2, but not to the 25 kDa CytA toxin of Bt var. israelensis. The cytolytic activity of these Bt var. kyushuensis toxins, like that of the latter two toxins, can be neutralized by incubation with liposomes containing phospholipids.FootnotesThis text was harvested from a scanned image of the original document using optical character recognition (OCR) software. As such, it may contain errors. Please contact the Royal Society if you find an error you would like to see corrected. Mathematical notations produced through Infty OCR. Next Article VIEW FULL TEXT DOWNLOAD PDF FiguresRelatedReferencesDetailsCited by Bravo A, López-Diaz J, Yamamoto T, Harding K, Zhao J, Mendoza G, Onofre J, Torres-Quintero M, Nelson M, Wu G, Sethi A and Soberón M (2018) Susceptible and mCry3A resistant corn rootworm larvae killed by a non-hemolytic Bacillus thuringiensis Cyt1Aa mutant, Scientific Reports, 10.1038/s41598-018-36205-6, 8:1, Online publication date: 1-Dec-2018. 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