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Hydroperoxide dependent O-dealkylation reactions catalyzed by liver microsomal cytochrome P450

1975; Elsevier BV; Volume: 62; Issue: 2 Linguagem: Inglês

10.1016/s0006-291x(75)80133-1

1090-2104

Anver D. Rahimtula, Peter J. O’Brien,

Drug Transport and Resistance Mechanisms

The organic hydroperoxide, cumene hydroperoxide was capable of supporting the O-dealkylation of a variety of methoxy and ethoxy compounds in the presence of hepatic microsomes — NADPH and molecular oxygen were not required for this reaction. Cytochrome P450 was implicated as the catalyst. Hemin and other hemoproteins were ineffective. The Km for the substrate was much higher with the cumene hydroperoxide dependent reaction than for the NADPH dependent reaction. The significance of the cumene hydroperoxide dependent reaction in elucidating the mechanism of cytochrome P450 reactions is discussed.