A Target Cell–Specific Activatable Fluorescence Probe for In vivo Molecular Imaging of Cancer Based on a Self-Quenched Avidin-Rhodamine Conjugate
2007; American Association for Cancer Research; Volume: 67; Issue: 6 Linguagem: Inglês
10.1158/0008-5472.can-06-3315
ISSN1538-7445
AutoresYukihiro Hama, Yasuteru Urano, Yoshinori Koyama, Mako Kamiya, Marcelino Bernardo, Ronald S. Paik, In‐Soo Shin, Chang H. Paik, Peter L. Choyke, Hisataka Kobayashi,
Tópico(s)Monoclonal and Polyclonal Antibodies Research
ResumoAbstract A target cell–specific activation strategy for improved molecular imaging of peritoneal implants has been proposed, in which fluorophores are activated only in living targeted cells. A current example of an activatable fluorophore is one that is normally self-quenched by attachment to a peptide backbone but which can be activated by specific proteases that degrade the peptide resulting in “dequenching.” In this study, an alternate fluorescence activation strategy is proposed whereby self-quenching avidin-rhodamine X, which has affinity for lectin on cancer cells, is activated after endocytosis and degradation within the lysosome. Using this approach in a mouse model of peritoneal ovarian metastases, we document target-specific molecular imaging of submillimeter cancer nodules with minimal contamination by background signal. Cellular internalization of receptor-ligand pairs with subsequent activation of fluorescence via dequenching provides a generalizable and highly sensitive method of detecting cancer microfoci in vivo and has practical implications for assisting surgical and endoscopic procedures. [Cancer Res 2007;67(6):2791–9]
Referência(s)