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Vacuum UV CD spectra of homopolymer duplexes and triplexes containing A.T or A.U base pairs

1991; Oxford University Press; Volume: 19; Issue: 9 Linguagem: Inglês

10.1093/nar/19.9.2275

1362-4962

Kenneth H. Johnson, Donald M. Gray, John C. Sutherland,

RNA Interference and Gene Delivery

Vacuum UV circular dichroism (CD) spectra were measured down to 174 nm for five homopolymers, five duplexes, and four triplexes containing adenine, uracil, and thymine.Near 190 nm, the CD bands of poly[d(A)] and poly[r(A)] were larger than the CD bands of the polypyrimidines, poly[d(T)], poly[d(U)], and poly[r(U)].Little change was observed in the 190 nm region upon formation of the duplexes (poly[d(A)*d(T)], poly[d(A) d(U)], poly[r(A) d(T)], poly[r(A) d(U)], and poly[r(A) * r(U)]) or upon formation of two of the triplexes (poly[d(T) * d(A) * d(T)] and poly[d(U) * d(A) -d(U)]).This showed that the purine strand had the same or a similar structure in these duplexes and triplexes as when free in solution.Both A* U and AaT base pairing induced positive bands at 177 and 202 nm.For three triplexes containing poly[d(A)], the formation of a triplex from a duplex and a free pyrimidine strand induced a negative band centered between 210 and 215 nm.The induction of a band between 210 and 215 nm indicated that these triplexes had aspects of the A conformation.