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Chemiluminescence Detector with a Serpentine Flow Cell

2008; American Chemical Society; Volume: 80; Issue: 24 Linguagem: Inglês

10.1021/ac801842q

1520-6882

Jessica M. Terry, Jacqui L. Adcock, Don C. Olson, Duane K. Wolcott, Cassie Schwanger, Lauren A. Hill, Neil W. Barnett, Paul S. Francis,

Advanced biosensing and bioanalysis techniques

We present a new chemiluminescence detector, with solution channels that have been machined into a Teflon disk and sealed with a sapphire window. The configuration of the flow cell can be conveniently modified by replacing the Teflon disk. A comparison of some existing and novel designs, using the chemiluminescence reaction of morphine with acidic potassium permanganate and the bioluminescence reaction of ATP with the commercially available "BacTiter-Glo" reagent, has revealed that a serpentine channel allows greater quantities of light to be captured than a spiral channel, due to more efficient mixing of the analyte and reagent solutions within the cell.