Intra-Mammary Duct Injections in the Study of Lactose Formation
1936; Elsevier BV; Volume: 19; Issue: 4 Linguagem: Inglês
10.3168/jds.s0022-0302(36)93060-6
ISSN1529-9066
AutoresWilliam R. Brown, W. E. Petersen, R. A. Gortner,
Tópico(s)Metastasis and carcinoma case studies
ResumoAs intravenous injections of sugars did not prove satisfactory in the production of a hyperglucemic condition, it was decided to study the effect of introducing sugars into the mammary gland upon the blood sugar level.Several literature references supported probable success of the idea.Jackson and Rothera (5) were the first to report a decrease in lactose content of milk if the removal milk was reintroduced into the udder.Davidson (2), studying the effect of incomplete removal of milk, found a subsequent lowering of the lactose content.Petersen and Rigor (6) injected lactose solutions into the udder and found tremendous reductions in lactose when removed twelve hours later.'1"~ PROBLEM The disapp.earanee of lactose observed by Petersen and Rigor indicated that absorption had occurred, so it was decided to perform similar injections and watch the milk, blood, and urine for changes in sugar content.As the right and left halves of the udder are completely separated, one gland can be used for injection and serve as a constantly draining reservoir.The other gland can be used for a study of the effect upon the milk.As no name has been given to this type of injection, it was decided to use the term "intramammary duct injection" throughout this paper. METHODS Injections.The intra-mammary duct injections were made through an ordinary teat canula and by means of gravity.The solutions were sterilized and buffered with sodium bicarbonate.~ampling.Blood, milk and urine samples were collected regularly.Mieturition was artificially stimulated whenever samples were to be collected.Analysis.Milk sugar was determined by the picrie acid method according to Bierman and Doan (1), blood sugar and urine sugar according to Shaffer-Hartman (7) on the filtrates prepared according to Folin-Wu (4).The unfermentable sugars were determined by the same technique after chloroform and yeast treated urine had stood at room temperature for about
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