CYTOTOXICITY OF MURINE PERITONEAL MACROPHAGES IN TUMOUR ALLOGRAFT IMMUNITY
1972; Wolters Kluwer; Volume: 14; Issue: 2 Linguagem: Inglês
10.1097/00007890-197208000-00012
ISSN1534-6080
AutoresWillem Den Otter, Robert Evans, Peter Alexander,
Tópico(s)Macrophage Migration Inhibitory Factor
ResumoSUMMARY Peritoneal macrophages from C57BL mice immunized with the DBA/2 lymphoma, SL2, were cytotoxic toward SL2 cells in vitro. The reaction required cell to cell contact and was not mediated via cytotoxins, complement-dependent antibody, or complementary activity in the supernatant fluids of challenged immune macrophage cultures. Cytotoxicity was immunologically specific involving antigenic recognition of the target cells by the immune macrophages, but it was not affected by the presence of antimouse γ-globulin. Trypsin abolished the cytotoxicity, which did not reappear if the treated macrophages were maintained in vitro. The kinetics of the cytotoxic reaction was studied by means of 51Cr release from the target cells, and the importance of the immune macrophage in allograft rejection is discussed
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