A high throughput screen to test the biocompatibility of water-miscible ionic liquids
2009; Royal Society of Chemistry; Volume: 11; Issue: 3 Linguagem: Inglês
10.1039/b815951c
ISSN1463-9270
AutoresMartin Rebroš, H. Q. Nimal Gunaratne, Jamie Ferguson, Kenneth R. Seddon, Gill Stephens,
Tópico(s)Innovative Microfluidic and Catalytic Techniques Innovation
ResumoThe Agar Diffusion test has been used for many years for testing antibiotic susceptibility in clinical laboratories. We wish to report that this simple, rapid test can also be applied to screen ionic liquids for toxicity to microorganisms. We screened 14 water miscible ionic liquids for toxicity for Clostridium butyricum using the test, and compared the results with EC50 values calculated from measurements of growth rates in the presence of the ionic liquids. The Agar Diffusion test could be used reliably to identify ionic liquids which were toxic to C. butyricum, with EC50 values below 0.034 M, and relatively non-toxic structures, with EC50 values above 0.063 M. Therefore, the Agar Diffusion test can be used with confidence to distinguish between toxic and biocompatible ionic liquids.
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