Codon Optimization of the Gene Encoding a Domain from Human Type 1 Neurofibromin Protein Results in a Threefold Improvement in Expression Level inEscherichia coli
1998; Elsevier BV; Volume: 12; Issue: 2 Linguagem: Inglês
10.1006/prep.1997.0825
ISSN1096-0279
AutoresRichard Hale, Gladstone Thompson,
Tópico(s)Bacterial Genetics and Biotechnology
ResumoAn internal domain from the human type 1 neurofibromin has previously been expressed inEscherichia colias a fusion with gluthathioneS-transferase (GST). The expression level of this protein was lower than expected and so a gene was constructed using the distribution of codons found in highly expressedE. coliproteins. Codons were assigned using a Microsoft Visual Basic computer program to give a distribution similar to those found in genes which are highly expressed inE. coli.The optimized gene was then cloned back into the same GST fusion plasmid and it was found that the expression of soluble protein had increased threefold.
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