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Effect of heparin on reactions at aminated polymer—blood interfaces

1969; Elsevier BV; Volume: 31; Issue: 2 Linguagem: Inglês

10.1016/0021-9797(69)90325-7

1095-7103

Leo Vroman, Ann L. Adams,

Advanced Sensor and Energy Harvesting Materials

Of two aminated polymers, tridodecyl-methyl ammonium chloride (TDMAC) and a polymer of 2-hydroxy-3-methacryloyloxypropyltrimethylammonium chloride (GMAC), the following properties were studied before and after the films were exposed to heparin. Film pressure on water, using a recording film balance; changes in optical thickness of films on oxidized silicon slices during exposure to plasma and antisera, using a recording ellipsometer; effects of films on coagulation, and on blood platelet adhesion. We found that onto GMAC films, normal, intact plasma deposits 100–150 Å of material that maintains its specific reactivity to antifibrinogen serum. Platelets adhered to GMAC-coated glass even in absence of fibrinogen or free calcium ions. Exposure of GMAC films to heparin caused an increase of about 15 Å and abolished the films' ability to attract either proteins or platelets; proteins adsorbed onto GMAC films were suddenly released after addition of heparin. Glass coated with GMAC, whether exposed to heparin or not, did not activate clotting and did not adsorb measurable amounts of Clotting Factor XII. Film pressure of GMAC on water was increased by heparin. TDMAC films were less attractive to proteins and platelets and were affected much less by adsorbed heparin.