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Broad spectrum identification of SUMO substrates in melanoma cells

2007; Wiley; Volume: 7; Issue: 13 Linguagem: Inglês

10.1002/pmic.200600971

1615-9861

Anand K. Ganesan, Yoonjung Kho, Sung Chan Kim, Yue Chen, Yingming Zhao, Michael A. White,

Glycosylation and Glycoproteins Research

Abstract Like phosphorylation, protein sumoylation likely represents a dynamic PTM to alter protein function in support of cell regulatory systems. The broad‐spectrum impact of transient or chronic engagement of signal transduction cascades on protein sumoylation has not been explored. Here, we find that epidermal growth factor (EGF) stimulation evokes a rapid alteration in small ubiquitin modifier (SUMO) target selection, while oncogene expression alters steady‐state SUMO‐protein profiles. A proteomic SUMO target analysis in melanoma cells identified proteins involved in cellular signaling, growth control, and neural differentiation.