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Interpretation of the osmotic behavior of sickle cell hemoglobin solutions: Different interactions among monomers and polymers

1998; Wiley; Volume: 45; Issue: 4 Linguagem: Inglês

10.1002/(sici)1097-0282(19980405)45

1097-0282

Jining Han, Judith Herzfeld,

Molecular spectroscopy and chirality

BiopolymersVolume 45, Issue 4 p. 299-306 Interpretation of the osmotic behavior of sickle cell hemoglobin solutions: Different interactions among monomers and polymers Jining Han, Jining Han Department of Chemistry and Keck Institute for Cellular Visualization, Brandeis University, Waltham, MA 02254-9110Search for more papers by this authorJudith Herzfeld, Corresponding Author Judith Herzfeld Department of Chemistry and Keck Institute for Cellular Visualization, Brandeis University, Waltham, MA 02254-9110Department of Chemistry and Keck Institute for Cellular Visualization, Brandeis University, Waltham, MA 02254-9110Search for more papers by this author Jining Han, Jining Han Department of Chemistry and Keck Institute for Cellular Visualization, Brandeis University, Waltham, MA 02254-9110Search for more papers by this authorJudith Herzfeld, Corresponding Author Judith Herzfeld Department of Chemistry and Keck Institute for Cellular Visualization, Brandeis University, Waltham, MA 02254-9110Department of Chemistry and Keck Institute for Cellular Visualization, Brandeis University, Waltham, MA 02254-9110Search for more papers by this author First published: 06 December 1998 https://doi.org/10.1002/(SICI)1097-0282(19980405)45:4 3.0.CO;2-GCitations: 7AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract It has long been known that a simple hard particle model quantitatively explains the osmotic properties of monomeric hemoglobin near its isoelectric point. However, we find that a hard particle model is not consistent with the osmotic properties of polymerized hemoglobin and that substantial soft repulsions are indicated. With allowance for different interactions among monomers and among polymers, a self-consistent quantitative fit to the experimental data is obtained. The results suggest that the decreasing “solubility” of deoxy sickle cell hemoglobin with increasing temperature from 20 to 37°C is due to weaker repulsions between polymers at higher temperatures rather than stronger polymerization. The temperature dependence of these variables indicates that the aggregation of monomers is enthalpically and entropically driven (the latter effect being stronger), while the approach of polymers toward each other is enthalpically disfavored and entropically favored (with the former dominating). In both cases, the entropic contribution suggests that water is released. © 1998 John Wiley & Sons, Inc. Biopoly 45: 299–306, 1998 Citing Literature Volume45, Issue45 April 1998Pages 299-306 RelatedInformation