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Parallel human genome analysis: microarray-based expression monitoring of 1000 genes.

1996; National Academy of Sciences; Volume: 93; Issue: 20 Linguagem: Inglês

10.1073/pnas.93.20.10614

1091-6490

Mark Schena, Dari Shalon, Renu A. Heller, Anwei Chai, P O Brown, Ronald W. Davis,

CRISPR and Genetic Engineering

Microarrays containing 1046 human cDNAs of unknown sequence were printed on glass with high-speed robotics. These 1.0-cm2 DNA "chips" were used to quantitatively monitor differential expression of the cognate human genes using a highly sensitive two-color hybridization assay. Array elements that displayed differential expression patterns under given experimental conditions were characterized by sequencing. The identification of known and novel heat shock and phorbol ester-regulated genes in human T cells demonstrates the sensitivity of the assay. Parallel gene analysis with microarrays provides a rapid and efficient method for large-scale human gene discovery.