Chemiluminometric l-lysine determination with immobilized lysine oxidase by flow-injection analysis
1993; Elsevier BV; Volume: 280; Issue: 2 Linguagem: Inglês
10.1016/0003-2670(93)85120-9
ISSN1873-4324
AutoresF. Preuschoff, U. Spohn, Ekkehard Weber, Kerstin Unverhau, Karsten Mohr,
Tópico(s)Biosensors and Analytical Detection
ResumoAn enzymatic flow-injection procedure for the determination of l-lysine was developed. A lysine oxidase reactor is combined with a fibre-optic hydrogen peroxide detector. Hydrogen peroxide detection is based on the peroxidase-catalysed luminol reaction. The chemiluminescent light is detected by a photomultiplier. l-Lysine can be determined in the range 10–1000 μM. A sampling rate of up to 90 h− can be achieved. The whole sensing assay works for more than 1 month. The double logarithmic graph of the peak signal height vs. the lysine concentration is linear, the slope being larger than unity (r2 = 0.991, n = 4).
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