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Artigo Acesso aberto Produção Nacional Revisado por pares
BIBTEX RIS

Intranasal vaccination with extracellular serine proteases of Leishmania amazonensis confers protective immunity to BALB/c mice against infection

2014; BioMed Central; Volume: 7; Issue: 1 Linguagem: Inglês

10.1186/1756-3305-7-448

ISSN

1756-3305

Autores

Herbert Leonel de Matos Guedes, Beatriz da Silva Costa, Suzana Passos Chaves, Daniel de Oliveira Gomes, Joshua D. Nosanchuk, Salvatore G. De-Simone, Bartira Rossi‐Bergmann,

Tópico(s)

Parasites and Host Interactions

Resumo

Previously, we demonstrated that unlike subcutaneous or intramuscular vaccination, intranasal vaccination of BALB/c mice with whole Leishmania amazonensis antigens leads to protection against cutaneous leishmaniasis. Here, the role of parasite serine proteases in the protective immunity was investigated. Serine Proteases were partially purified from both soluble (LaSP-Sol) and extracellular (LaSP-Ex) Leishmania amazonensis promastigote extracts by aprotinin-agarose chromatography. BALB/c mice were intranasally immunized with LaSP-Sol and LaSP-Ex prior to infection with L. amazonensis. LaSP-Ex but not LaSP-Sol vaccination led to significantly smaller lesions and parasite burdens as compared with non-vaccinated controls. Protection was accompanied by systemic Th1 polarization with increased IFN-γ and decreased IL-4 and IL-10 splenic production. Likewise, increased production of IFN-γ, IL-12 and IL-4 concomitant with decreased TGF-β and TNF-α was locally observed in the infected footpad. This study indicates that extracellular serine proteases of L. amazonensis are strong candidates for a more defined intranasal vaccine against cutaneous leishmaniasis.

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