RELEASE OF ESTRADIOL FROM FETAL BOVINE SERUM BY RAT THYMUS, SPLEEN, KIDNEY, LUNG AND LUNG MACROPHAGE CULTURES
1982; Oxford University Press; Volume: 110; Issue: 6 Linguagem: Inglês
10.1210/endo-110-6-2213
ISSN1945-7170
AutoresHYGIE REYNOLDS, Paul Nathan, Laxmi Srivastava, Evelyn V. Hess,
Tópico(s)Effects and risks of endocrine disrupting chemicals
ResumoHigh levels of estradiol(E2) were detected by radioimmunoassay(RIA) in supernatants of rat thymus and spleen, kidney, lung and lung macrophages cultured in the presence of fetal bovine serum(FBS). A competitive inhibitor of E2synthesis had no effect on the production of E2 by thymic cultures. Acid hydrolysis of estrogen conjugates in FBS released enough free E2 to account for the high level found in culture supernatants. Removal of free estrogens and estrogen conjugates by charcoal stripping the serum before addition to media reduced thymic culture E2 release to undetectable levels. It appears likely that E2 is produced in cultures of rat tissues by hydrolysis of estrogen conjugates in FBS.
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