Synthesis of thermo- and acid-stable novel oligosaccharides by using dextransucrase with high concentration of sucrose
2006; Elsevier BV; Volume: 40; Issue: 5 Linguagem: Inglês
10.1016/j.enzmictec.2006.08.017
ISSN1879-0909
AutoresEun-Seong Seo, Seung‐Hee Nam, Hee-Kyung Kang, Jae-Yong Cho, Hee-Seon Lee, Hwa‐Won Ryu, Doman Kim,
Tópico(s)Enzyme Catalysis and Immobilization
ResumoA method is presented for synthesizing thermo-, acid-stable oligosaccharides (TASO) from sucrose (2.5–4 M) using a dextransucrase prepared from Leuconostoc mesenteroides B-512FMCM. The degree of polymerization (DP) of oligosaccharides synthesized was from 2 to 11. TASO resisted hydrolysis of its glycosidic linkages at 140 °C and pH 6.0 for 1 h. It was stable at pH's ranging from 2 to 4 at 120 °C. These oligosaccharides effectively inhibited the formation of insoluble glucan, the growth and acid production of Streptococcus sobrinus. However, it stimulated the growth of probiotic organisms such as Bifidobaterium sp. TASO potentially can be used as sweeteners for the food and beverages where thermo- and acid-stable properties are required and as potential inhibitors of dental caries.
Referência(s)