Produção Nacional
Revisado por pares
Efeito do anti-TNF-α em implantes endometriais no peritônio de ratas
2011; Brazilian College of Surgeons; Volume: 38; Issue: 4 Linguagem: Inglês
10.1590/s0100-69912011000400011
ISSN1809-4546
AutoresWilliam Kondo, Eduardo Andreazza Dal Lago, Lúcia de Noronha, Márcia Olandoski, Paulo Gustavo Kotze, Vivian Ferreira do Amaral,
Tópico(s)Gynecological conditions and treatments
ResumoTo evaluate the effect of anti-TNF-α in the treatment of endometrial implants in the peritoneum of rats.Endometrial implants were surgically induced in 120 female Wistar-Albino rats. The animals were randomly divided into four groups. Group C (n = 36) received an intraperitoneal injection of 0.2 ml of saline. Group L (n = 41) received a subcutaneous injection of 1mg/kg of leuprolide. Group I5 (n = 20) received a subcutaneous injection of 5mg/kg of monoclonal anti-tumor necrosis factor (TNF) a (infliximab). Group I10 (n = 20) received a subcutaneous injection of 10mg/kg of infliximab. The rats were sacrificed after 21 days to assess the size of the implants and the expression of TNF.Treatment with leuprolide (group L) promoted an absolute reduction in the surface area of the implant when compared with group C (+14 mm vs. 0mm, p = 0.013) and group I10 (+14 mm vs. +5 Mm, p = 0.018). Likewise, a percentage reduction of surface area of the implant was observed comparing group L with group C (+33.3% vs. 0%, p = 0.005) and group I10 (+33.3% vs. +18.3%, p = 0.027). Treatment with infliximab was not able to decrease the surface area of the implants when compared with group C. The expression of TNF-α in groups L, I5 and I10 was lower than in group C (505.6 mm(2) vs. 660.5 mm(2) vs. 317.2 mm(2) vs. 2519.3 mm(2), respectively; p <0.001).The anti-TNF-α therapy reduced the expression of TNF-α in endometriotic implants, but did not reduce the surface area of the lesion.
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