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Proanthocyanidin from grape seeds inactivates the PI3-kinase/PKB pathway and induces apoptosis in a colon cancer cell line

2007; Elsevier BV; Volume: 258; Issue: 1 Linguagem: Inglês

10.1016/j.canlet.2007.08.020

1872-7980

Anna‐Mart Engelbrecht, Mary Mattheyse, B. Ellis, Ben Loos, Mark Thomas, R. Smith, Susanna Peters, Carine Smith, Kathryn H. Myburgh,

Genomics, phytochemicals, and oxidative stress

The aim of this investigation was to evaluate the chemopreventative/antiproliferative potential of a grape seed proanthocyanidin extract (GSPE) against colon cancer cells (CaCo2 cells) and to investigate its mechanism of action. GSPE (10–100 μg/ml) significantly inhibited cell viability and increased apoptosis in CaCo2 cells, but did not alter viability in the normal colon cell line (NCM460). The increased apoptosis observed in GSPE-treated CaCo2 cells correlated with an attenuation of PI3-kinase (p110 and p85 subunits) and decreased PKB Ser473 phosphorylation. GSPE might thus exert its beneficial effects by means of increased apoptosis and suppression of the important PI3-kinase survival-related pathway.