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Promoter-Selective Properties of the TBP-Related Factor TRF1

2000; American Association for the Advancement of Science; Volume: 288; Issue: 5467 Linguagem: Inglês

10.1126/science.288.5467.867

1095-9203

Michael C. Holmes, Robert Tjian,

CRISPR and Genetic Engineering

The TATA-binding protein (TBP)–related factor 1 (TRF1) is expressed in a tissue-restricted fashion during Drosophila embryogenesis and may serve as a promoter-specific recognition factor that can replace TBP in regulating transcription. However, bona fide target promoters that would preferentially respond to TRF1 have remained elusive. Polytene chromosome staining, chromatin immunoprecipitation, direct messenger RNA analysis, and transient cotransfection assays identified the Drosophila gene tudor as containing a TRF1-responsive promoter. Reconstituted in vitro transcription reactions and deoxyribonuclease I footprinting assays confirmed the ability of TRF1 to bind preferentially and direct transcription of the tudor gene from an alternate promoter. Thus, metazoans appear to have evolved gene-selective and tissue-specific components of the core transcription machinery to regulate gene expression.