Portal de Periódicos da CAPES

Hansschlegelia plantiphila gen. nov. sp. nov., a new aerobic restricted facultative methylotrophic bacterium associated with plants

2007; Elsevier BV; Volume: 30; Issue: 6 Linguagem: Inglês

10.1016/j.syapm.2007.03.001

1618-0984

Ekaterina Ivanova, Н. В. Доронина, Yuri A. Trotsenko,

Biofuel production and bioconversion

A new genus, Hansschlegelia, and a new species, Hansschlegelia plantiphila, are proposed for three strains of methanol-utilizing bacteria isolated from lilac buds (strain S1T), linden buds (strain S2) and blue spruce needles (strain S4), which were selected in winter at −17 °C. These bacteria are aerobic, Gram-negative, colorless, non-motile short rods that multiply by binary fission and employ the ribulose bisphosphate (RuBP) and the serine pathways for C1 assimilation. The strains have a limited number of growth substrates and use methanol, methylamine, formate, CO2/H2 and glycerol as carbon and energy sources. Only strain S1T grows with ethanol and inulin. The strains are neutrophilic and mesophilic, and synthesize phytohormones (auxins and cytokinins) and vitamin B12. Their major cellular fatty acids are saturated C16:0, straight-chain, unsaturated C18:1ω7 and cyclopropane C19 cyc acids. The main ubiquinone is ubiquinone-10 (Q-10). The dominant phospholipids are phosphatidylethanolamine, phosphatidylcholine and diphosphatidylglycerol (cardiolipin). The DNA G+C content is 68.5±0.2 mol%. The strains share almost identical 16S rRNA gene sequences, a high DNA–DNA hybridization value (72–86%) and represent a novel lineage of autotrophic methanol-utilizing bacteria within the Alphaproteobacteria. Collectively, these strains comprise a new genus and species H. plantiphila gen. nov., sp. nov., with strain S1T (VKM B-2347T, NCIMB 14035T) as the type strain.