Are aberrant BCR-ABL transcripts more common than previously thought?
2000; Wiley; Volume: 111; Issue: 4 Linguagem: Inglês
10.1046/j.1365-2141.2000.02471.x
ISSN1365-2141
AutoresG. A. Wilson, Elisabeth Vandenberghe, Rebecca C. Pollitt, David C. Rees, Anne Goodeve, I. R. Peake, John T. Reilly,
Tópico(s)Acute Lymphoblastic Leukemia research
ResumoWe report the use of multiplex polymerase chain reaction (PCR), using 4% polyacrylamide gel electrophoresis (PAGE) for the detection of BCR-ABL transcripts in Philadelphia-positive disease. Three out of 50 cases [two out of 37 chronic myeloid leukaemia (CML), one out of 13 acute lymphoblastic leukaemia (ALL)] possessed rare breakpoints; an e19a2 and e13a3 in CML and an e1a3 in the ALL. We suggest that multiplex PCR using 4% PAGE and optimized for smaller transcript detection may lead to a higher detection rate of rare BCR-ABL breakpoints. Multiplex PCR, however, failed to distinguish e13a2 from e1a3 transcripts. Finally, the presence of e13a3 in CML supports the view that abl exon 2 sequences are unnecessary for the pathogenesis of 'classic' CML.
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