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In vivo nonlinear spectral imaging in mouse skin

2006; Optica Publishing Group; Volume: 14; Issue: 10 Linguagem: Inglês

10.1364/oe.14.004395

1094-4087

Jonathan A. Palero, Henriëtte S. de Bruijn, A. van der Ploeg–van den Heuvel, Henricus J. C. M. Sterenborg, Hans C. Gerritsen,

Spectroscopy Techniques in Biomedical and Chemical Research

We report on two-photon autofluorescence and second harmonic spectral imaging of live mouse tissues. The use of a high sensitivity detector and ultraviolet optics allowed us to record razor-sharp deep-tissue spectral images of weak autofluorescence and short-wavelength second harmonic generation by mouse skin. Real-color image representation combined with depth-resolved spectral analysis enabled us to identify tissue structures. The results show that linking nonlinear deep-tissue imaging microscopy with autofluorescence spectroscopy has the potential to provide important information for the diagnosis of skin tissues.