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Imaging of hydroperoxide and hydrogen peroxide‐scavenging substances by photon emission

2001; Wiley; Volume: 16; Issue: 5 Linguagem: Inglês

10.1002/bio.663

1522-7243

Yumiko Yoshiki, Tetsuo Iida, Yoshinobu Akiyama, Kazuyoshi Ōkubo, Hitoshi Matsumoto, Michikatsu Sato,

Metabolomics and Mass Spectrometry Studies

Abstract An imaging system for the hydroperoxide and hydrogen donor was developed by photon emission from the reactive oxygen species–hydrogen donor–KHCO 3 –MeCHO system. Photon intensity (luminance, cd/m 2 ) showed a linear correlation with the concentration of some hydroperoxides [hydrogen peroxide (H 2 O 2 ), tert ‐butyl hydroperoxide and methyl ethyl ketone peroxide] and that of hydrogen donors (catechins and anthocyanins). The linear relationship between photon intensity and concentration was observed in polyphenol‐rich samples, such as teas, berries and currants. Photon intensity from the H 2 O 2 /polyphenol‐rich sample–KHCO 3 –MeCHO system corresponded with H 2 O 2 ‐scavenging activity, rather than with polyphenol contents. It is possible to classify hydrogen donor species according to absorption features using spectral analysis (gallic acid, E max 645 nm, catechins, E max 610 nm; anthocyanins, E max 690–700 nm). This method is a simple and sensitive detection system for hydroperoxide‐ and H 2 O 2 ‐scavenging substances. Copyright © 2001 John Wiley & Sons, Ltd.