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The effect of doxorubicin (adriamycin) on cytoplasmic microtubule system in cardiac cells

1987; Elsevier BV; Volume: 19; Issue: 11 Linguagem: Inglês

10.1016/s0022-2828(87)80352-8

1095-8584

S W Rabkin, Paul S. Sunga,

Diamond and Carbon-based Materials Research

The objective of this study was to determine whether the antineoplastic agent doxorubicin (adriamycin) affects the microtubule system in isolated cardiac cells. Embryonic chick heart cultures were exposed to adriamycin concentrations of 40, 80, or 200 μm for 3, 12, or 24 h before fixation prior to immunoflourescence. Adriamycin produced marked distortions in the normal microtubule system. This was a function of adriamycin concentration and the duration of the exposure to adriamycin. Even when adriamycin was removed from the medium for a 12 h period, cells treated with adriamycin showed abnormalities of the microtubule system. The effect of adriamycin on the microtubule system extends to its reassembly after colchicine. Cells allowed to recover after colchicine in the presence of adriamycin (40 μm) showed impairment in microtubule regeneration. These data indicate that the microtubule system in cardiac myocytes is affected by adriamycin both by damaging microtubule structure and by impairing microtubule reassembly. The objective of this study was to determine whether the antineoplastic agent doxorubicin (adriamycin) affects the microtubule system in isolated cardiac cells. Embryonic chick heart cultures were exposed to adriamycin concentrations of 40, 80, or 200 μm for 3, 12, or 24 h before fixation prior to immunoflourescence. Adriamycin produced marked distortions in the normal microtubule system. This was a function of adriamycin concentration and the duration of the exposure to adriamycin. Even when adriamycin was removed from the medium for a 12 h period, cells treated with adriamycin showed abnormalities of the microtubule system. The effect of adriamycin on the microtubule system extends to its reassembly after colchicine. Cells allowed to recover after colchicine in the presence of adriamycin (40 μm) showed impairment in microtubule regeneration. These data indicate that the microtubule system in cardiac myocytes is affected by adriamycin both by damaging microtubule structure and by impairing microtubule reassembly.