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Isolation and characterisation of KP34—a novel φKMV-like bacteriophage for Klebsiella pneumoniae

2011; Springer Science+Business Media; Volume: 90; Issue: 4 Linguagem: Inglês

10.1007/s00253-011-3149-y

1432-0614

Zuzanna Drulis‐Kawa, Paweł Mackiewicz, Agata Kęsik-Szeloch, Ewa Maciaszczyk-Dziubińska, Beata Weber‐Dąbrowska, Agata Dorotkiewicz-Jach, Daria Augustyniak, Grażyna Majkowska-Skrobek, Tomasz Bocer, Joanna Empel, Andrew M. Kropinski,

Mycobacterium research and diagnosis

Bacteriophage KP34 is a novel virus belonging to the subfamily Autographivirinae lytic for extended-spectrum β-lactamase-producing Klebsiella pneumoniae strains. Its biological features, morphology, susceptibility to chemical and physical agents, burst size, host specificity and activity spectrum were determined. As a potential antibacterial agent used in therapy, KP34 molecular features including genome sequence and protein composition were examined. Phylogenetic analyses and clustering of KP34 phage genome sequences revealed its clear relationships with "phiKMV-like viruses". Simultaneously, whole-genome analyses permitted clustering and classification of all phages, with completely sequenced genomes, belonging to the Podoviridae.