Application of reductive cleavage in the structural investigation of the antigenic polysaccharides of Aspergillus fumigatus and Penicillium digitatum with respect to the determination of the ring size of the galactose moieties
1994; Elsevier BV; Volume: 23; Issue: 1 Linguagem: Inglês
10.1016/0144-8617(94)90084-1
ISSN1879-1344
AutoresPetra Mischnick, Gerhard A. De Ruiter,
Tópico(s)Glycosylation and Glycoproteins Research
ResumoThe structures of the antigenic extracellular polysaccharides of Aspergillus fumigatus and Penicillium digitatum have been investigated with respect to the linkage positions and ring size of the galactose moieties by the reductive-cleavage technique and by standard methylation analysis. 6-O-Linked and 5,6-di-O-linked galactofuranose residues were identified as new structural features. While 4-O-linked galactopyranose is also present in the extracellular polysaccharide of A. fumigatus, no significant amounts could be determined in the extracellular polysaccharide of P. digitatum. Results were proved by a two-step degradation procedure involving selective reductive cleavage of the galactofuranosyl side chains using a mixture of trimethylsilyl trichloroacetate and borontrifluoride etherate (5:1) as mild Lewis acid, and triethylsilane followed by subsequent remethylation, complete degradation, and GLC-MS analysis.
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