Molecular-level dengue fever diagnostic devices made out of paper
2013; Royal Society of Chemistry; Volume: 13; Issue: 14 Linguagem: Inglês
10.1039/c3lc50135c
ISSN1473-0197
AutoresShih-Jie Lo, Shih-Chun Yang, Da‐Jeng Yao, Jiann‐Hwa Chen, Wu‐Chun Tu, Chao‐Min Cheng,
Tópico(s)Advanced biosensing and bioanalysis techniques
ResumoThis paper describes the development of nucleic acid detection in paper using a combination of commercial fluorescent probes and DNA ladders, and provides us with a better understanding of the interactions between double-stranded DNA (the amplified products in this study), fiber structures in paper, and fluorescent probes. The amplified products (the reverse-transcription and amplification of dengue virus serotype-2 RNA via RT-LAMP) in this study were subsequently fluorescently labeled in paper-based test zones (on our paper-based diagnostic device), thus fluorescent probes were used to perform the diagnosis of dengue fever, specific to serotype-2.
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