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XERODERMA PIGMENTOSUM COMPLEMENTATION GROUP F: MORE ASSIGNMENTS AND REPAIR CHARACTERISTICS

1985; Wiley; Volume: 41; Issue: 5 Linguagem: Inglês

10.1111/j.1751-1097.1985.tb03538.x

1751-1097

Yoshisada Fujiwara, Yoshihiko Uehara, Masamitsu Ichihashi, Kazue Nishioka,

CRISPR and Genetic Engineering

Abstract— The specific heterodikaryon complementation method enabled us to assign three patients with mild xeroderma pigmentosum (XP) symptoms (XP25KO, XP27KO, XP28KO) to complementation group F. UV‐induced unscheduled DNA synthesis (UDS) remained unnormalized in the heterodikaryons between either of the above three XP strains and the reference group F XP3YO. All these particular XP strains as well as XP3YO exhibited an equally low level of10–15% UDS by a 3 h [ 3 H]‐thymidine labeling following 10 J/m 2 254 nm UV, while they attained 60% UDS of normal at an extended time of 25 h. The present group F strains were 3 and 1.5 times as sensitive to the lethal effect of UV as normal and XP group E cells, respectively, based on the mean lethal dose ( Do ) comparison. Normal cells had the biphasic time‐UDS kinetics of early rapid and late slow repair. Characteristically, however, all of the present group F strains were defective in only early rapid repair, but normally proficient in slow repair.