Artigo Revisado por pares

The Fluorescent Reaction between Berberine and DNA and the Fluorometry of DNA

1998; Elsevier BV; Volume: 60; Issue: 1 Linguagem: Inglês

10.1006/mchj.1998.1633

ISSN

1095-9149

Autores

Wen‐You Li, Zuhong Lu,

Tópico(s)

Drug Transport and Resistance Mechanisms

Resumo

Determination of DNA with berberine(BER) as a fluorescent probe was developed, based on the large fluorescence enhancement of BER in the presence of DNA and the poor fluorescence yield of BER in the absence of DNA. The calibration graph was linear in the range 0–20 μg/ml CT DNA. The limit of detection was 12 ng/ml CT DNA.

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