Discovery of a New Family of Dieckmann Cyclases Essential to Tetramic Acid and Pyridone-Based Natural Products Biosynthesis
2015; American Chemical Society; Volume: 17; Issue: 3 Linguagem: Inglês
10.1021/ol5036497
ISSN1523-7060
AutoresChun Gui, Qinglian Li, Xuhua Mo, Xiangjing Qin, Junying Ma, Jianhua Ju,
Tópico(s)Genomics and Phylogenetic Studies
ResumoBioinformatic analyses indicate that TrdC, SlgL, LipX2, KirHI, and FacHI belong to a group of highly homologous proteins involved in biosynthesis of actinomycete-derived tirandamycin B, streptolydigin, α-lipomycin, kirromycin, and factumycin, respectively. However, assignment of their biosynthetic roles has remained elusive. Gene inactivation and complementation, in vitro biochemical assays with synthetic analogues, point mutations, and phylogenetic tree analyses reveal that these proteins represent a new family of Dieckmann cyclases that drive tetramic acid and pyridone scaffold biosynthesis.
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