Inhibition of platelet aggregation by S ‐nitroso‐cysteine via cGMP‐independent mechanisms: evidence of inhibition of thromboxane A 2 synthesis in human blood platelets
1999; Wiley; Volume: 442; Issue: 2-3 Linguagem: Inglês
10.1016/s0014-5793(98)01633-0
ISSN1873-3468
AutoresDimitrios Tsikas, Milos Ikic, Kathrin S. Tewes, Manfred Raida, Jürgen C. Frölich,
Tópico(s)Synthesis and Catalytic Reactions
ResumoS ‐Nitroso‐cysteine (SNC), a putative endothelium‐derived relaxing factor, potently inhibited collagen‐ and arachidonic acid‐induced platelet aggregation (IC 50 =100 nM) and thromboxane A 2 (TxA 2 ) synthesis of human blood platelets. ODQ, a selective inhibitor of the soluble guanylyl cyclase, inhibited SNC‐induced formation of cGMP but did not reverse inhibition by SNC of collagen‐ and arachidonic acid‐induced platelet aggregation. Combination of ODQ with SQ‐29548, a specific platelet TxA 2 receptor antagonist, did not modify the antiaggregatory action of SNC. Our study shows that SNC inhibits platelet aggregation by cGMP‐independent mechanisms that may involve inhibition of TxA 2 synthesis in human platelets.
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