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Systematic evaluation of distribution of transgene expression after adenovirus-mediated gene transfer to the transplanted heart

1998; Springer Science+Business Media; Volume: 11; Issue: 5 Linguagem: Inglês

10.1007/s001470050160

1432-2277

Carlo Pellegrini, Timothy O’Brien, John Yap, Anders Jeppsson, Henry D. Tazelaar, Christopher G.A. McGregor,

RNA Interference and Gene Delivery

In the transplantation setting, the study and potential treatment of acute and chronic rejection by means of gene therapy will require widespread transgene expression in the donor organ. The distribution of transgene expression after adenovirus-mediated gene transfer via the coronary vasculature in a model of abdominal heterotopic heart transplantation in syngeneic rats (n = 6) was evaluated at 1 week. Reporter gene expression was evenly distributed in the base, the midventricle, and the apex of the transplanted hearts. This study demonstrates that intracoronary administration of the adenoviral vector to the donor heart results in widespread transgene expression.