Expression of glycolipid blood group antigens in single human kidneys: Change in antigen expression of rejected ABO incompatible kidney grafts
1993; Elsevier BV; Volume: 44; Issue: 6 Linguagem: Inglês
10.1038/ki.1993.381
ISSN1523-1755
AutoresAmi Ulfvin, Annika E. Bäcker, Henrik Clausen, Sen‐itiroh Hakomori, Lennart Rydberg, Bo E. Samuelsson, Michael E. Breimer,
Tópico(s)Glycosylation and Glycoproteins Research
ResumoTotal neutral glycolipid fractions were separated into molecular species on thin-layer chromatography plates and detected by immunostaining with monoclonal anti-blood group antibodies. Blood group A antigens based on type 1, 2, 3 and 4 carbohydrate core saccharides were present in kidneys of A1 and A1B individuals. Blood group A2 individuals expressed only small amounts of A antigen compared to A1 individuals especially of the type 3 and 4 compounds. Kidneys from non-secretor individuals contained less A antigen compared to secretor individuals, and in both groups a variation in the antigen expression between single individuals was noted. Blood group A type 2 and 3 (which is an extension of A type 2) antigens were present both as basic 6 and 9 sugar structures as well as extended saccharide chains migrating in the 8 to 11 sugar interval. In contrast, the type 1 chain based A and Lewis antigens were only present as their basic 5 to 7 sugar chains, and no elongated structures were found. Four cases of A2 kidneys initially transplanted into O recipients and removed after 5, 12, 21 days and 4 years, respectively, were also analyzed. Two of these kidneys, originating from the same donor, showed a difference in A antigen expression. The kidney functioning for four years (lost due to chronic rejection) completely lacked X antigen with five sugar residues (present in all other individuals) and contained a large amount of A antigens.(ABSTRACT TRUNCATED AT 250 WORDS)
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