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Parylene-A coated microplate for covalent immobilization of proteins and peptides

2009; Elsevier BV; Volume: 353; Issue: 1-2 Linguagem: Inglês

10.1016/j.jim.2009.11.015

1872-7905

Byung-Jin Jeon, Moo-Hwan Kim, Jae‐Chul Pyun,

Monoclonal and Polyclonal Antibodies Research

Various types of microplates made of polystyrene have been widely used for immunoassays. A new microplate suitable for the covalent immobilization of proteins and peptides was developed by thermal deposition of amino-modified parylene (parylene-A) on the microplate. The primary amine groups of the parylene-A was exploited for the covalent coupling of proteins and peptides. The optical transmittance at the wavelength of 400–500 nm was estimated to be suitable for the application to immunoassays. The immobilization efficiency of the parylene-A coated microplate was demonstrated to be far improved in comparison to the conventional microplate by using horseradish peroxidase (HRP), anti-HRP antibody and a peptide with 9-residues as model biomolecules.