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Rhodnius prolixus Malpighian tubule's aquaporin expression is modulated by 5‐hydroxytryptamine

2004; Wiley; Volume: 57; Issue: 3 Linguagem: Inglês

10.1002/arch.20017

1520-6327

Sabrina V. Martini, Regina Coeli dos Santos Goldenberg, Antonio F. Fortes, Antônio Carlos Campos de Carvalho, D Falkenstein, Marcelo M. Morales,

Physiological and biochemical adaptations

Abstract The purpose of the present study was to detect the presence of an aquaporin‐like water channel, a member of the major intrinsic protein (MIP) family, in the Malpighian tubule (MT) of the hematophagous insect Rhodnius prolixus , which excrete a large bulk of fluid after a massive blood meal, and its possible regulation by 5‐hydroxytryptamine (5‐HT). Reverse transcription polymerase chain reaction (RT‐PCR) and Southern blots of cDNA was obtained from adult R. prolixus MT poly (A) + RNA. Employing degenerate primers corresponding to the NPA (amino acid sequence motifs repeats Asn‐Pro‐Ala) highly conserved domain of amino acids sequences of all members of the MIP gene family, we were able to identify a 365‐base pair PCR product. The R. prolixus MT mRNA expression of this water transporter is increased in the animal after blood meal and in tubules treated with 5‐hydroxytryptamine or cAMP. The up‐regulated expression of MT MIP mRNA after a blood meal is probably due to the action of 5‐hydroxytryptamine via a cyclic AMP dependent pathway. Arch. Insect Biochem. Physiol. 57:133–141, 2004. © 2004 Wiley‐Liss, Inc.